Noninvasive Real-Time Monitoring of Liver-Stage Development of Bioluminescent Plasmodium Parasites
Identifieur interne : 001784 ( Main/Exploration ); précédent : 001783; suivant : 001785Noninvasive Real-Time Monitoring of Liver-Stage Development of Bioluminescent Plasmodium Parasites
Auteurs : Agnes Mwakingwe ; Li-Min Ting ; Sarah Hochman ; John Chen ; Photini Sinnis [États-Unis] ; Kami Kim [États-Unis]Source :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2009.
Abstract
BackgroundThe morbidity and mortality associated with malaria are heightened because of the spread of drug-resistant parasites and the lack of an effective vaccine. Plasmodium liver stages are the targets of new chemotherapeutics and vaccines, but there are limited tools available to study this stage in vivo MethodsTo overcome this obstacle, we developed a method with which to study Plasmodium liver stages by means of bioluminescent imaging (BLI) of the rodent malaria parasite Plasmodium yoelii. We created a P. yoelii YM strain (PyLuc) that stably expresses firefly luciferase driven by a constitutive promoter ResultsUsing BLI, we performed imaging of the Plasmodium liver stages of mice infected with PyLuc sporozoites and monitored parasite dissemination during blood-stage infection. Because PyLuc luciferase activity is proportional to the number of parasites, BLI can be used to quantify the effect of drugs on liver-stage development. Moreover, using BLI, we demonstrated that immunization with blood-stage parasites confers partial protective immunity against the development of liver stages ConclusionsBLI is a noninvasive technique that is useful for screening potential drugs and candidate vaccines with which to combat malaria. The prospect of cross-stage protective immunity increases the number of avenues to be explored in the development of an effective vaccine against malaria
Url:
DOI: 10.1086/606115
Affiliations:
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Plasmodium liver stages are the targets of new chemotherapeutics and vaccines, but there are limited tools available to study this stage in vivo MethodsTo overcome this obstacle, we developed a method with which to study Plasmodium liver stages by means of bioluminescent imaging (BLI) of the rodent malaria parasite Plasmodium yoelii. We created a P. yoelii YM strain (PyLuc) that stably expresses firefly luciferase driven by a constitutive promoter ResultsUsing BLI, we performed imaging of the Plasmodium liver stages of mice infected with PyLuc sporozoites and monitored parasite dissemination during blood-stage infection. Because PyLuc luciferase activity is proportional to the number of parasites, BLI can be used to quantify the effect of drugs on liver-stage development. Moreover, using BLI, we demonstrated that immunization with blood-stage parasites confers partial protective immunity against the development of liver stages ConclusionsBLI is a noninvasive technique that is useful for screening potential drugs and candidate vaccines with which to combat malaria. The prospect of cross-stage protective immunity increases the number of avenues to be explored in the development of an effective vaccine against malaria</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><noCountry><name sortKey="Chen, John" sort="Chen, John" uniqKey="Chen J" first="John" last="Chen">John Chen</name><name sortKey="Hochman, Sarah" sort="Hochman, Sarah" uniqKey="Hochman S" first="Sarah" last="Hochman">Sarah Hochman</name><name sortKey="Mwakingwe, Agnes" sort="Mwakingwe, Agnes" uniqKey="Mwakingwe A" first="Agnes" last="Mwakingwe">Agnes Mwakingwe</name><name sortKey="Ting, Li Min" sort="Ting, Li Min" uniqKey="Ting L" first="Li-Min" last="Ting">Li-Min Ting</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Sinnis, Photini" sort="Sinnis, Photini" uniqKey="Sinnis P" first="Photini" last="Sinnis">Photini Sinnis</name></region><name sortKey="Kim, Kami" sort="Kim, Kami" uniqKey="Kim K" first="Kami" last="Kim">Kami Kim</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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